Cloning and Expression of VP2 Gene of Infectious Bursal Disease Virus in Eukaryotic Cells

Authors

  • Hamid Galehdari Department of Biology, Faculty of Sciences, Shahid Chamran University of Ahvaz, P.O. Box 65355-141, Ahvaz, I.R. Iran
  • Masoud Reza Seyfiabad Shapouri Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, P.O. Box 65355-145, Ahvaz, I.R. Iran
  • Mohammad Roayaei Department of Biology, Faculty of Sciences, Shahid Chamran University of Ahvaz, P.O. Box 65355-141, Ahvaz, I.R. Iran
  • Roozbeh Hushiarian Department of Biology, Faculty of Sciences, Shahid Chamran University of Ahvaz, P.O. Box 65355-141, Ahvaz, I.R. Iran
Abstract:

Infectious bursal disease (IBD) is an economically important viral disease of chickens with worldwide distribution which suppresses the immune system of young chickens. VP2 is the major host-protective protein of infectious bursal disease virus (IBDV). The encoding region of VP2 protein was PCR amplified from a plasmid containing a cDNA fragment of large genomic segment of IBDV, strain D78. This region of 1356 bp was inserted into a eukaryotic expression plasmid, pCDNA4, under the control of human cytomegalovirus (hCMV) immediate early enhancer and promoter. Plasmid DNA was transfected into COS-7 cell line and transient expression of VP2 from the constructed plasmid was characterized by dot blotting with a polyclonal antibody to IBDV.

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Journal title

volume 5  issue 4

pages  246- 250

publication date 2007-10-01

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